研究業績 Publications
伊川正人特任教授の大阪大学での
研究業績はこちら
2022
*equal contribution #corresponding author
1. Ozawa M, Emori C, #Ikawa M.
CRISPR/Cas9-mediated highly efficient gene targeting in embryonic stem cells for developing gene-
manipulated mouse models.
Journal of Visulalized Experiments 2022:186
https://pubmed.ncbi.nlm.nih.gov/36094255/
2. Ozawa M, Emori C,# Ikawa M.
Gene targeting in mouse embryonic stem cells via CRISPR/Cas9 ribonucleoprotein (RNP) mediated genome editing.
In: Hatada I (ed) Genome Editing in Animals – Methods and Protocols 2nd edition. 2022
3. Hensel JA, Nicholas SE, Jellison ER, Kimble AL, Menoret A, Ozawa M, Rodriguez-Oquendo A, Vella AT, #Murphy PA.
Splice Factor Polypyrimidine tract-binding protein 1 (Ptbp1) is Required for Immune Priming of the Endothelium in Atherogenic
Disturbed Flow Conditions.
Proc Natl Acad Sci U S A 2022;119:e2122227119.
https://pubmed.ncbi.nlm.nih.gov/35858420/
4. Hoang T, Kim DW, Appel H, Pannullo NA, Ozawa M, Zheng S, Yu M, Peachey NS, Kim J, #Blackshaw S.
Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina.
Cell Reports 2022;39:110849.
https://pubmed.ncbi.nlm.nih.gov/35705053/
5. Shimada-Takayama Y, Yasuda T, Ukai T, Taguchi J, Ozawa M, Sankoda N Ohta S, #Yamada Y.
Generation of mice for evaluating endogenous p16Ink4a protein expression.
Biochem Biophys Res Commun 2022;599:43-50.
https://pubmed.ncbi.nlm.nih.gov/35168063/
6. Hirano M, So Y, Tsunekawa S, Kabata M, Ohta S, Sagara H, Sankoda N, Taguchi J, Yamada Y, Ukai T, Kato M, Nakamura J, Ozawa M,
Yamamoto T, #Yamada Y.
MYCL-mediated reprogramming expands pancreatic insulin-producing cells.
Nat Metab 2022;4:254-268.
https://pubmed.ncbi.nlm.nih.gov/35145326/
7. Taguchi J, Shibata H, Kabata M, Kato M, Fukuda K, Tanaka A, Ohta S, Ukai T, Mitsunaga K, Yamada Y, Nagaoka S, Yamasawa S,
Ohnishi K, Woltjen K, Ushiku T, Ozawa M, Saitou M, Shinkai Y, Yamamoto T, #Yamada Y.
DMRT1-mediated reprogramming drives development of cancer resembling human germ cell tumors with features of totipotency
Nat Commun 2021;12:5041.
https://pubmed.ncbi.nlm.nih.gov/34413299/
8. #Ozawa M, Taguchi J, Katsuma K, Ishikawa-Yamauchi Y, Kikuchi M, Sakamoto R, Yamada Y, Ikawa M.
Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice.
Sci Rep 2022;12: 21558.
https://www.nature.com/articles/s41598-022-26107-z
9. Iida-Norita R, Miyata H, Kaneda Y, Emori C, Noda T, Nakagawa T, #Matzuk MM, #Ikawa M.
Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs.
Andrology. 2022 Dec 4. doi: 10.1111/andr.13359.
https://pubmed.ncbi.nlm.nih.gov/36464740/
10. Sakurai N, #Fujihara Y, Kobayashi K, Ikawa M.
CRISPR/Cas9-mediated disruption of lipocalins, Ly6g5b, and Ly6g5c causes male sub-fertility in mice.
Andrology. 2022 Nov 25. doi: 10.1111/andr.13350.
https://pubmed.ncbi.nlm.nih.gov/36428102/
11. Yokoyama M, Fujita T, Kadonosawa Y, Tatara Y, Motooka D, Ikawa M, Fujii H, #Yokoayama Y.
Development of transgenic mice overexpressing mouse carbonyl reductase 1.
Mol Biol Rep. 2022 Nov 9. doi: 10.1007/s11033-022-07994-x.
https://pubmed.ncbi.nlm.nih.gov/36352178/
12. #Fujihara Y, Kobayashi K, Abbasi F, Endo T, Yu Z, Ikawa M, Matzuk MM.
PDCL2 is essential for sperm acrosome formation and male fertility in mice.
Andrology. 2022 Oct 24. doi: 10.1111/andr.13329.
https://pubmed.ncbi.nlm.nih.gov/36278277/
13. *Tang S, *Lu Y, Skinner WM, Sanyal M, Lishko PV, #Ikawa M, #Kim PS.
Human sperm TMEM95 binds eggs and facilitates membrane fusion.
Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2207805119. doi: 10.1073/pnas.2207805119. Epub 2022 Sep 26.
https://pubmed.ncbi.nlm.nih.gov/36161911/
14. Irie M, Itoh J, Matsuzawa A, Ikawa M, Kiyonari H, Kihara M, Suzuki T, Hiraoka Y, #Ishino F, #Kaneko-Ishino T.
Retrovirus-derived RTL5 and RTL6 genes are novel constituents of the innate immune system in the eutherian brain.
Development. 2022 Sep 15;149(18):dev200976. doi: 10.1242/dev.200976. Epub 2022 Sep 27.
https://pubmed.ncbi.nlm.nih.gov/36162816/
15. Nozawa K, Fujihara Y, Devlin DJ, Deras RE, Kent K, Larina IV, Umezu K, Yu Z, Sutton CM, Ye Q, Dean LK, Emori C, Ikawa M, Garcia TX, #Matzuk MM.
The testis-specific E3 ubiquitin ligase RNF133 is required for fecundity in mice.
BMC Biol. 2022 Jul 13;20(1):161. doi: 10.1186/s12915-022-01368-2.
https://pubmed.ncbi.nlm.nih.gov/35831855/
16. Oura S, Hino T, Satoh T, Noda T, Koyano T, Isotani A, Matsuyama M, Akira S, Ishiguro KI, #Ikawa M.
Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice.
PLoS Genet. 2022 Jun 1;18(6):e1010241. doi: 10.1371/journal.pgen.1010241.
https://pubmed.ncbi.nlm.nih.gov/35648791/
17. Kaneda Y, #Miyata H, Shimada K, Oyama Y, Iida-Norita R, #Ikawa M.
IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice.
Dev Biol. 2022 May 23:S0012-1606(22)00099-9. doi: 10.1016/j.ydbio.2022.05.011.
https://pubmed.ncbi.nlm.nih.gov/35618043/
18. Oyama Y, #Miyata H, Shimada K, Larasati T, Fujihara Y, #Ikawa M
TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility.
Reprod Med Biol. 2022 May 23;21(1):e12467. doi: 10.1002/rmb2.12467. eCollection Jan-Dec 2022.
https://pubmed.ncbi.nlm.nih.gov/35619658/
19. Noda T, Blaha A, Fujihara Y, Gert KR, Emori C, Deneke VE, Oura S, Panser K, Lu Y, Berent S, Kodani M, Cabrera-Quio LE, #Pauli A, #Ikawa M.
Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish.
Commun Biol. 2022 Apr 7;5(1):332. doi: 10.1038/s42003-022-03289-w.
https://pubmed.ncbi.nlm.nih.gov/35393517/
20. Hiramatsu Y, Suzuki K, Nishida T, Onoda N, Satoh T, Akira S, Ikawa M, Ikeda H, Kamei J, Derouiche S, Tominaga M, #Horiguchi Y.
The Mechanism of Pertussis Cough Revealed by the Mouse-Coughing Model.
mBio. 2022 Mar 31;e0319721. doi: 10.1128/mbio.03197-21.
https://pubmed.ncbi.nlm.nih.gov/35357202/
21. El Hussien MA, Tsai CY, Satouh Y, Motooka D, Okuzaki D, Ikawa M, #Kikutani H, #Sakakibara S.
Corrigendum to: Multiple tolerance checkpoints restrain affinity maturation of B cells expressing the germline precursor of a lupus patient-derived anti-dsDNA antibody in knock-in mice.
Int Immunol. 2022 Mar 25;34(4):225. doi: 10.1093/intimm/dxac007.
https://pubmed.ncbi.nlm.nih.gov/35348696/
22. Yamauchi Y, Matsumura T, Bakse J, Holmlund H, Blanchet G, Carrot E, Ikawa M, #Ward MA.
Loss of mouse Y chromosome gene Zfy1 and Zfy2 leads to spermatogenesis impairment, sperm defects, and infertility.
Biol Reprod. 2022 Mar 16;ioac057. doi: 10.1093/biolre/ioac057.
https://pubmed.ncbi.nlm.nih.gov/35293998/
23. Mise S, #Matsumoto A, Shimada K, Hosaka T, Takahashi M, Ichihara K, Shimizu H, Shiraishi C, Saito D, Suyama M, Yasuda T, Ide T, Izumi Y, Bamba T, Kimura-Someya T, Shirouzu M, Miyata H, Ikawa M, #Nakayama KI.
Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.
Nat Commun. 2022 Feb 28;13(1):1071. doi: 10.1038/s41467-022-28677-y.
https://pubmed.ncbi.nlm.nih.gov/35228556/
24. Hirata T, Kobayashi A, Furuse T, Yamada I, Tamura M, Tomita H, Tokoro Y, Ninomiya A, Fujihara Y, Ikawa M, Maeda Y, Murakami Y, #Kizuka Y, #Kinoshita T.
Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
J Biol Chem. 2022 Feb 10;101720. doi: 10.1016/j.jbc.2022.101720.
https://pubmed.ncbi.nlm.nih.gov/35151686/
25. #Miyata H, Oyama Y, Kaneda Y, #Ikawa M.
The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.
Exp Anim. 2022 Feb 9;71(1):46-52. doi: 10.1538/expanim.21-0082. Epub 2021 Sep 15.
https://pubmed.ncbi.nlm.nih.gov/34526446/
26. Matsumura T, Noda T, Satouh Y, Morohoshi A, Yuri S, Ogawa M, Lu Y, Isotani A, #Ikawa M.
Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma in Mice and Rats.
Front Cell Dev Biol. 2022 Jan 12;9:810118. doi: 10.3389/fcell.2021.810118. eCollection 2021.
https://pubmed.ncbi.nlm.nih.gov/35096839/
27. Hwang JY, Wang H, Lu Y, Ikawa M, #Chung JJ.
C2cd6-encoded CatSperτ targets sperm calcium channel to Ca 2+ signaling domains in the flagellar membrane.
Cell Rep. 2022 Jan 18;38(3):110226. doi: 10.1016/j.celrep.2021.110226. Epub 2022 Jan 7.
https://pubmed.ncbi.nlm.nih.gov/34998468
2021
*equal contribution #corresponding author
-
#Yoshida K, Fujita SI, Isotani A, Kudo T, Takahashi S, Ikawa M, Shiba D, Shirakawa M, Muratani M, Ishii S.
Intergenerational effect of short-term spaceflight in mice.
iScience. 2021 Jun 25;24(7):102773. doi: 10.1016/j.isci.2021.102773. eCollection 2021 Jul 23.
https://pubmed.ncbi.nlm.nih.gov/34278272/
-
Oyama Y, #Miyata H, Shimada K, Fujihara Y, Tokuhiro K, Garcia TX, Matzuk MM, #Ikawa M.
CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice.
Asian J Androl. 2021 Jul 16. doi: 10.4103/aja.aja_63_21. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/34290169/
-
Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, #Ikawa M.
Precise CAG repeat contraction in a Huntington’s Disease mouse model is enabled by gene editing with SpCas9-NG.
Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w.
https://pubmed.ncbi.nlm.nih.gov/34163001/
-
#Monsivais D, Nagashima T, Prunskaite-Hyyryläinen R, Nozawa K, Shimada K, Tang S, Hamor C, Agno JE, Chen F, Masand RP, Young SL, Creighton CJ, DeMayo FJ, Ikawa M, Lee SJ, #Matzuk MM.
Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis.
Nat Commun. 2021 Jun 7;12(1):3386. doi: 10.1038/s41467-021-23571-5.
https://pubmed.ncbi.nlm.nih.gov/34099644/
-
Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro KI, #Ikawa M.
KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice.
PLoS Genet. 2021 May 7;17(5):e1009412. doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.
https://pubmed.ncbi.nlm.nih.gov/33961623/
-
Qu Y, Chen Q, Guo S, Ma C, Lu Y, Shi J, Liu S, Zhou T, Noda T, Qian J, Zhang L, Zhu X, Lei X, Cao Y, Li W, Li W, Plachta N, Matzuk MM, Ikawa M, #Duan E, #Zhang Y, #Wang H.
Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization.
Protein Cell. 2021 Mar 1. doi: 10.1007/s13238-021-00825-y. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/33649997/
-
Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, #Matzuk MM, #Ikawa M.
ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility.
Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118.
https://pubmed.ncbi.nlm.nih.gov/33536340/
-
*#Fukuda E, *Tanaka H, Yamaguchi K, Takasaka M, Kawamura Y, Okuda H, Isotani A, Ikawa M, Shapiro VS, Tsuchida J, Okada Y, Tsujimura A, Miyagawa Y, Fukuhara S, Kawakami Y, Wada M, Nishimune Y, Goshima N.
Identification and characterization of the antigen recognized by the germ cell mAb TRA98 using a human comprehensive wet protein array.
Genes Cells. 2021 Mar;26(3):180-189. doi: 10.1111/gtc.12832.
https://pubmed.ncbi.nlm.nih.gov/33527666/
-
Zhou Z, Okamoto K, Onodera J, Hiragi T, Andoh M, Ikawa M, Tanaka KF, Ikegaya Y, Koyama R.
Astrocytic cAMP modulates memory via synaptic plasticity.
Proc Natl Acad Sci U S A. 2021 Jan 19;118(3):e2016584118.
https://pubmed.ncbi.nlm.nih.gov/33452135/
-
#Kim CR, Noda T, Okada Y, #Ikawa M, #Baek SH.
Protocol for isolation of spermatids from mouse testes.
STAR Protoc. 2021 Jan 8;2(1):100254.
https://pubmed.ncbi.nlm.nih.gov/33490974/
11. Kaiwen Liu, Ryota Sato, Takuma Shibata, Ryosuke Hiranuma, Tatjana Reuter, Ryutaro Fukui, Yun Zhang, Takeshi Ichinohe, Manabu
Ozawa, Nobuaki Yoshida, Eicke Latz , #Kensuke Miyake
Skewed endosomal RNA responses from TLR7 to TLR3 in RNase T2-deficient macrophages
International Immunology. 2021 Jun 23 doi: 10.1093/intimm/dxab033.
https://pubmed.ncbi.nlm.nih.gov/34161582/
12. Tadashi Yamamuro, Shuhei Nakamura, Yu Yamano, Tsutomu Endo, Kyosuke Yanagawa,, Ayaka Tokumura, Takafumi Matsumura,
Kiyonori Kobayashi, Hideto Mori, Yusuke Enokidani, Gota Yoshida, Hitomi Imoto, Tsuyoshi Kawabata, Maho Hamasaki, Akiko Kuma,
Sohei Kuribayashi,Kentaro Takezawa, Yuki Okada, Manabu Ozawa, Shinichiro Fukuhara, Takashi Shinohara, Masahito Ikawa, and #Tamotsu Yoshimori
Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function
Plos Genet. 2021 Aug 5. doi: 10.1371/journal.pgen.1009688
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341604/
2020
Omori S, Wang TW, Johmura1 Y, Kanai T, Nakano Y, Kido T, Susaki EA, Nakajima T, Shichino S, Ueha S, Ozawa M, Yokote K, Kumamoto S, Nishiyama A, Sakamoto T, Yamaguchi K, Hatakeyama S, Shimizu E, Katayama K, Yamada Y, Yamazaki S, Miyoshi C, Yanagisawa M, Ueno H, Imoto S, Furukawa Y, Yoshida N, Matsushima K, Ueda HR, Miyajima A, NakanishiN M.
Generation of a p16 reporter mouse and its use to characterize and target p16 high cells in vivo.
Cell Metabolism 2020;32:814-828.
Senoo M, Hozoji H, Ishikawa-Yamauchi Y, Takijiri T, Ohta S, Ukai T, Kabata M, Yamamoto T, Yamada Y, Ikawa M, Ozawa M.
RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in spermatogonia and maintains spermatogenesis in concert with Nanos3.
J Reprod Dev 2020;66:459-467.
Kiyozumi D, Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren KM, Horie M, Okabe M, Matzuk MM, Ikawa M.
NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility.
Science 2020;368:1132-1135.
Miyata H, Abbasi F, Visconti PE, Ikawa M.
CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in mice.
Biol Reprod. 2020 Oct 29;103(5):912-914.
*Liu C, *Miyata H, *Gao Y, *Sha Y, *Tang S, *Xu Z, Whitfield M, Patrat C, Wu H, Dulioust E, Tian S, Shimada K, Cong J, Noda T, Li H, Morohoshi A, Cazin C, Kherraf ZE, Arnoult C, Jin L, He X, Ray PF, Cao Y, Touré A, Zhang F, Ikawa M. *:equal contribution.
Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility.
Am J Hum Genet. 2020 Aug 6;107(2):330-341.
*Park S, *Shimada K, Fujihara Y, Xu Z, Shimada K, Larasati T, Pratiwi P, Matzuk RM, Devlin DJ, Yu Z, Garcia TX, Matzuk MM,
Ikawa M. *:equal contribution.
CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity.
Biol Reprod. 2020 Aug 4;103(2):195-204.
Miyata H, Morohoshi A, Ikawa M.
Analysis of the sperm flagellar axoneme using gene-modified mice.
Exp Anim. 2020 Nov 12;69(4):374-381.
*Sun J, *Lu Y, Nozawa K, Xu Z, Morohoshi A, Castaneda JM, Noda T, Miyata H, Abbasi F, Shawki HH, Takahashi S, Devlin DJ, Yu Z, Matzuk RM, Garcia TX, Martin MM, Ikawa M. *:equal contribution.
CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproduction.
Biol Reprod. 2020 Aug 4;103(2):183-194.
*Xu Z, *Miyata H, Kaneda Y, Castaneda JM, Lu Y, Morohoshi A, Yu Z, Matzuk MM, Ikawa M.
CIB4 Is Essential for the Haploid Phase of Spermatogenesis in Mice.
Biol Reprod. 2020 Aug 4;103(2):235-243.
*Larasati T, *Noda T, Fujihara Y, Shimada K, Tobita T, Yu Z, Matzuk MM, Ikawa M. *:equal contribution
Tmprss12 is required for sperm motility and uterotubal junction migration in mice.
Biol Reprod. 2020 Aug 4;103(2):254-263.
*Noda T, *Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk MM, Ikawa M.*:equal contribution
Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
PNAS.2020;117(21):11493‐11502.
*Kobayashi K, *Endo T, Matsumura T, Lu Y, Yu Z, Matzuk MM, Ikawa M.*:equal contribution
Prss55 but Not Prss51 Is Required for Male Fertility in Mice.
Biol Reprod. 2020 Aug 4;103(2):223-234.
Fujihara Y, Lu Y, Noda T, Oji A, Larasati T, Kojima-Kita K, Yu Z, Matzuk RM, Matzuk MM, Ikawa M.
Spermatozoa Lacking Fertilization Influencing Membrane Protein (FIMP) Fail to Fuse With Oocytes in Mice.
PNAS. 2020 Apr 28;117(17):9393-9400.
*Abbasi F, *Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, Ikawa M. *:equal contribution
CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
Cells. 2020;9(4):E821. Published 2020 Mar 28.
Miyata H, Shimada K, Morohoshi A, Oura S, Matsumura T, Xu Z, Oyama Y, Ikawa M.
Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa.
FASEB J. 2020 Apr;34(4):5389-5400.
Kiyozumi D, Mori M, Kodani M, Ikawa M.
Genetic mutation of Frem3 does not cause Fraser syndrome in mice.
Exp Anim. 2020 Jan 29;69(1):104-109.
Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, Ikawa M.
Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice.
PLoS Genet. 2020 Jan 21;16(1):e1008585.
Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, Ikawa M.
TESMIN, METALLOTHIONEIN-LIKE 5, is required for spermatogenesis in mice.
Biol Reprod. 2020;102(4):975–983.
2019
Noda T, Sakurai N, Nozawa K, Kobayashi S, Devlin DJ, Matzuk MM, Ikawa M.
Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice.
Andrology. 2019; 7(5): 644–653
Tobita T, Kiyozumi D, Muto M, Noda T, Ikawa M.
Lvrn expression is not critical for mouse placentation.
J Reprod Dev. 2019; 65(3): 239–244.
Shimada K, Kato H, Miyata H, Ikawa M.
Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis.
J Reprod Dev. 2019; 65(2): 155–162.
Matsumura T, Endo T, Isotani A, Ogawa M, Ikawa M.
An Azoospermic Factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility.
J Reprod Dev. 2019;65(2):121-128.
Senoo M, Takijiri T, Yoshida N, Ozawa M, Ikawa M.
PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia.
J Reprod Dev 2019;65:37–46.
Komura S, Ito K, Ohta S, Ukai T, Kabata M, Itakura F, Semi F, Matsuda Y, Hashimoto K, Shibata H, Sone M, Jo N, Sekiguchi K, Ohno T, Akiyama H, Shimizu K, Woltjen K, Ozawa M, Toguchi J, Yamamoto T, Yamada Y.
Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas.
Nat Commun 2019;10:3999.
Sasanuma H, Ozawa M, Yoshida N.
RNA binding protein Ptbp1 is essential for BCR-mediated antibody production.
Int Immunol 2019;31:158-166.
*Matsumura T, *Noda T, Muratani M, Okada R, Yamane M, Isotani A, Kudo T, Takahashi S, Ikawa M.
*: equal contribution
Male mice, caged in the International Space Station for 35 days, sire healthy offspring.
Sci Rep. 2019;9(1):13733.
Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi D, Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX,
Ikawa M.
CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice.
Biol Reprod. 2019;101(2):501-511.
Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, Ikawa M.
Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity.
Biol Reprod.2019 Apr 19;100(4):1035-1045
Oura S, Miyata H, Noda T, Shimada K, Matsumura T, Morohoshi A, Isotani A, Ikawa M.
Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice.
Exp Anim. 2019 Feb 26;68(1):25-34.
2018
Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, Ikawa M. Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity.Biol Reprod. 2018 Nov 18.
Fujihara Y, Oji A, Kojima-Kita K, Larasati T, Ikawa M. Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice.J Cell Sci. 2018 Oct 8;131(19).
Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, Ikawa M. RSPH6A is required for sperm flagellum formation and male fertility in mice.J Cell Sci. 2018 Oct 11;131(19).
Oura S, Miyata H, Noda T, Shimada K, Matsumura T, Morohoshi A, Isotani A, Ikawa M. Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice. Exp Anim. 2018 Aug 9.
Nozawa K, Satouh Y, Fujimoto T, Oji A, Ikawa M. Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice. Sci Rep. 2018 Jan 22;8(1):1315.
Sasanuma S, Ozawa M, Yoshida N. The RNA binding protein Ptbp1 is essential for BCR-mediated antibody production. Int Immunol (accepted)
2017
Fujihara Y, Oji A, Larasati T, Kojima-Kita K, Ikawa M
Human globozoospermia-related gene Spata16 is required for spermformation revealed by CRISPR/Cas9-mediated mouse models.
International Journal of Molecular Sciences, 18(10): 2208 (2017)
Castaneda JM, Hua R, Miyata H, Oji A, Guo Y, Cheng Y, Zhou T, Guo X, Cui Y, Shen B, Wang Z, Hu Z, Zhou Z, Sha J, Prunskaite-Hyyrylainen R, Yu Z, Ramirez-Solis R, Ikawa M, Matzuk MM, Liu M. TCTE1 is a conserved component of the dynein regulatory complex and is required for motilityand metabolism in mouse spermatozoa.Proc Natl Acad Sci U S A. pii: 201621279 (2017)
Tribulo P, Moss JI, Ozawa M, Jiang Z, Tian C, Hansen PJ. WNT regulation of embryonic development likely involves pathways independent of nuclear CTNNB1. Reproduction 153:405-419 (2017)
2016
Zhang X, Chen H, Wu X, Kodani A, Fan J, Doan R, Ozawa M, Ma J, Yoshida N, Reiter JF, Black DL, Kharchenko PV, Sharp PA, Walsh CA. Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex. Cell 166:1147-1162 (2016)
Ozawa M, Sakatani M, Dobbs K, Kannampuzha-Francis J, Hansen PJ. Regulation of gene expression in the bovine blastocyst by colony stimulating factor 2. BMC Research Notes 9:250 (2016)
Ozawa M, Fukuda T, Sakamoto R, Honda H, Yoshida N. The Histone Demethylase FBXL10 Regulates the Proliferation of Spermatogonia and Ensures Long-Term Sustainable Spermatogenesis in Mice. Biol Reprod 94:1-11 (2016)
Yang QE, Ozawa M, Zhang K, Johnson S, Ealy AD. The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development. Reprod Fertil Dev 28:482-490 (2016)
2014
Ozawa M, Kawakami E, Sakamoto R, Shibasaki T, Goto A, Yoshida N. Development of FGF2-dependent pluripotent stem cells showing naive state characteristics from murine preimplantation inner cell mass. Stem Cell Res 13:75-87 (2014)
Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N. The histone demethylases Fbxl11 plays an essential role in embryonic development by repressing cell-cycle regulators. Mech Devel 135:31-42 (2014)
Nakai M, Ozawa M, Maedomari N, Noguchi J, Kaneko H, Ito J, Onishi A, Kashiwazaki N, Kikuchi K. Delay in cleavage of porcine embryos after intracytoplasmic sperm injection (ICSI) shows poorer embryonic development. J Reprod Dev 60:256-259 (2014)
2013
Ozawa M, Yang QE, Ealy AD. The expression of fibroblast growth factor receptors during early bovine conceptus development and pharmacological analysis of their actions on trophoblast growth in vitro. Reproduction 145:191-201 (2013)
Sakatani M, Bonilla L, Dobbs KB, Block J, Ozawa M, Shanker S, Yao J, Hansen PJ. Changes in the transcriptome of morula-stage bovine embryos caused by heat shock: relationship to developmental acquisition of thermotolerance. Reprod Biol Endocrinol 11:3 (2013)
Dobbs KB, Khan FA, Sakatani M, Moss JI, Ozawa M, Ealy AD, Hansen PJ. Regulation of pluripotency of inner cell mass and growth and differentiation of trophectoderm of the bovine embryo by colony stimulating factor 2. Biol Reprod 89:1-10 (2013)
2012
Ozawa M, Sakatani M, Yao J, Shanker S, Yu F, Yamashita R, Wakabayashi S, Nakai K, Dobbs KB, Sudano MJ, Farmerie WG, Hansen PJ. Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst. BMC Dev Biol 12:33 (2012)
Ozawa M, Sakatani M, Hankowski KE, Terada N, Dobbs KB, Hansen PJ. Importance of culture conditions during the morula-to-blastocyst period on capacity of inner cell-mass cells of bovine blastocysts for establishment of self-renewing pluripotent cells. Theriogenology 78:1243-1251 (2012)
Nunomura S, Shimada S, Kametani Y, Yamada Y, Yoshioka M, Suemizu H, Ozawa M, Itoh T, Kono A, Suzuki R, Tani K, Ando K, Yagita H, Ra C, Habu S, Satake M, Sasaki E. Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset). Int Immunol 24:593-603 (2012)
2011
Bonilla AQ, Oliveira LJ, Ozawa M, Newsom EM, Lucy MC. Hansen PJ. Developmental changes in thermoprotective actions of insulin-like growth factor-1 on the preimplantation bovine embryo. Mol Cell Endocrinol 332:170-179 (2011)
Bonilla AQ, Ozawa M, Hansen PJ. Timing and dependence upon mitogen-activated protein kinase signaling for pro-developmental actions of insulin-like growth factor 1 on the preimplantation bovine embryo. Growth Horm IGF Res 2:107-111 (2011)
Ozawa M, Hansen PJ. A novel method for purification of inner cell mass and trophectoderm cells from blastocysts using magnetic activated cell sorting. Fertil Steril 95:799-802 (2011)
Thompson IM, Ozawa M, Bubolz JW, Yang Q, Dahl GE. Bovine luteal prolactin receptor expression: potential involvement in regulation of progesterone during the estrous cycle and pregnancy. J Anim Sci 89:1338-1346 (2011)
Yang QE, Fields SD, Zhang K, Ozawa M, Johnson SE, Ealy AD. Fibroblast Growth Factor 2 Promotes Primitive Endoderm Development in Bovine Blastocyst Outgrowths. Biol Reprod 85:946-953 (2011)
2010
Dang-Nguyen TQ, Kikuchi K, Somfai, Ozawa M, Nakai M, Maedomari Viet-Linh N, Kanai Y, Nguyen BX, Nagai T. Evaluation of developmental competence of in vitro-produced porcine embryos based on the timing, pattern and evenness of the first cleavage and the onset of the second cleavage. J Reprod Dev 56:593-600 (2010)
Maedomari N, Kikuchi K, Nagai T, Fahrudin M, Kaneko H, Noguchi J, Nakai M, Ozawa M, Somfai T, Nguyen LV, Ito J, Kashiwazaki N. Nuclear Replacement of In Vitro-Matured Porcine Oocytes by a Serial Centrifugation and Fusion Method. Reprod Domest Anim 45:659-665 (2010)
Nakai M, Kaneko H, Somfai T, Maedomari N, Ozawa M, Noguchi J, Ito J, Kashiwazaki N, Kikuchi K. Production of viable piglets for the first time using sperm derived from ectopic testicular xenografts. Reproduction 139:331–335 (2010)
Ozawa M, Nagai T, Somfai T, Nakai M, Maedomari N, Miyazaki H, Kaneko H, Noguchi J, Kikuchi K. Cumulus cell-enclosed oocytes acquire a capacity to synthesize GSH by FSH stimulation during in vitro maturation in pigs. J Cell Physiol 222:294-301 (2010)
Somfai T, Noguchi J, Kaneko H, Nakai M, Ozawa M, Kashiwazaki N, Egerszegi I, Rátky J, Nagai T, Kikuchi K. Production of good-quality porcine blastocysts by in vitro fertilization of follicular oocytes vitrified at the germinal vesicle stage. Theriogenology 73:147-156 (2010)