​研究業績    Publications

​伊川正人特任教授の大阪大学での

研究業績はこちら

2021

*equal contribution #corresponding author

  1. #Yoshida K, Fujita SI, Isotani A, Kudo T, Takahashi S, Ikawa M, Shiba D, Shirakawa M, Muratani M, Ishii S.
    Intergenerational effect of short-term spaceflight in mice.
    iScience. 2021 Jun 25;24(7):102773. doi: 10.1016/j.isci.2021.102773. eCollection 2021 Jul 23.
    https://pubmed.ncbi.nlm.nih.gov/34278272/

     

  2. Oyama Y, #Miyata H, Shimada K, Fujihara Y, Tokuhiro K, Garcia TX, Matzuk MM, #Ikawa M.
    CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice.
    Asian J Androl. 2021 Jul 16. doi: 10.4103/aja.aja_63_21. Online ahead of print.
    https://pubmed.ncbi.nlm.nih.gov/34290169/

     

  3. Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, #Ikawa M.
    Precise CAG repeat contraction in a Huntington’s Disease mouse model is enabled by gene editing with SpCas9-NG.
    Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w.
    https://pubmed.ncbi.nlm.nih.gov/34163001/

     

  4. #Monsivais D, Nagashima T, Prunskaite-Hyyryläinen R, Nozawa K, Shimada K, Tang S, Hamor C, Agno JE, Chen F, Masand RP, Young SL, Creighton CJ, DeMayo FJ, Ikawa M, Lee SJ, #Matzuk MM.
    Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis.
    Nat Commun. 2021 Jun 7;12(1):3386. doi: 10.1038/s41467-021-23571-5.
    https://pubmed.ncbi.nlm.nih.gov/34099644/

     

  5. Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro KI, #Ikawa M.
    KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice.
    PLoS Genet. 2021 May 7;17(5):e1009412. doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.
    https://pubmed.ncbi.nlm.nih.gov/33961623/

     

  6. Qu Y, Chen Q, Guo S, Ma C, Lu Y, Shi J, Liu S, Zhou T, Noda T, Qian J, Zhang L, Zhu X, Lei X, Cao Y, Li W, Li W, Plachta N, Matzuk MM, Ikawa M, #Duan E, #Zhang Y, #Wang H.
    Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization.
    Protein Cell. 2021 Mar 1. doi: 10.1007/s13238-021-00825-y. Online ahead of print.
    https://pubmed.ncbi.nlm.nih.gov/33649997/

     

  7. Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, #Matzuk MM, #Ikawa M.
    ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility.
    Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118.
    https://pubmed.ncbi.nlm.nih.gov/33536340/

     

  8. *#Fukuda E, *Tanaka H, Yamaguchi K, Takasaka M, Kawamura Y, Okuda H, Isotani A, Ikawa M, Shapiro VS, Tsuchida J, Okada Y, Tsujimura A, Miyagawa Y, Fukuhara S, Kawakami Y, Wada M, Nishimune Y, Goshima N.
    Identification and characterization of the antigen recognized by the germ cell mAb TRA98 using a human comprehensive wet protein array.
    Genes Cells. 2021 Mar;26(3):180-189. doi: 10.1111/gtc.12832.
    https://pubmed.ncbi.nlm.nih.gov/33527666/

     

  9. Zhou Z, Okamoto K, Onodera J, Hiragi T, Andoh M, Ikawa M, Tanaka KF, Ikegaya Y, Koyama R.
    Astrocytic cAMP modulates memory via synaptic plasticity.
    Proc Natl Acad Sci U S A. 2021 Jan 19;118(3):e2016584118. 
    https://pubmed.ncbi.nlm.nih.gov/33452135/

     

  10. #Kim CR, Noda T, Okada Y, #Ikawa M, #Baek SH.
    Protocol for isolation of spermatids from mouse testes.
    STAR Protoc. 2021 Jan 8;2(1):100254. 
    https://pubmed.ncbi.nlm.nih.gov/33490974/   

11. Kaiwen LiuRyota Sato, Takuma ShibataRyosuke HiranumaTatjana ReuterRyutaro FukuiYun ZhangTakeshi IchinoheManabu 

      OzawaNobuaki YoshidaEicke Latz , #Kensuke Miyake 

      Skewed endosomal RNA responses from TLR7 to TLR3 in RNase T2-deficient macrophages

      International Immunology. 2021 Jun 23  doi: 10.1093/intimm/dxab033.

      https://pubmed.ncbi.nlm.nih.gov/34161582/

12. Tadashi Yamamuro, Shuhei Nakamura, Yu Yamano, Tsutomu Endo, Kyosuke Yanagawa,, Ayaka Tokumura, Takafumi Matsumura, 

      Kiyonori Kobayashi, Hideto Mori, Yusuke Enokidani, Gota Yoshida, Hitomi Imoto, Tsuyoshi Kawabata, Maho Hamasaki, Akiko Kuma,

      Sohei Kuribayashi,Kentaro Takezawa, Yuki Okada, Manabu Ozawa, Shinichiro Fukuhara, Takashi Shinohara, Masahito Ikawa, and          #Tamotsu Yoshimori

      Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function

      Plos Genet. 2021 Aug 5. doi: 10.1371/journal.pgen.1009688

      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341604/

2020

Omori S, Wang TW, Johmura1 Y, Kanai T, Nakano Y, Kido T, Susaki EA, Nakajima T, Shichino S, Ueha S, Ozawa M, Yokote K, Kumamoto S, Nishiyama A, Sakamoto T, Yamaguchi K, Hatakeyama S, Shimizu E, Katayama K, Yamada Y, Yamazaki S, Miyoshi C, Yanagisawa M, Ueno H, Imoto S, Furukawa Y, Yoshida N, Matsushima K, Ueda HR, Miyajima A, NakanishiN M.

Generation of a p16 reporter mouse and its use to characterize and target p16 high cells in vivo.

Cell Metabolism 2020;32:814-828.

Senoo M, Hozoji H, Ishikawa-Yamauchi Y, Takijiri T, Ohta S, Ukai T, Kabata M, Yamamoto T, Yamada Y, Ikawa M, Ozawa M.

RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in spermatogonia and maintains spermatogenesis in concert with Nanos3.

J Reprod Dev 2020;66:459-467.

Kiyozumi D, Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren KM, Horie M, Okabe M, Matzuk MM, Ikawa M.

NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility.

Science 2020;368:1132-1135.

Miyata H, Abbasi F, Visconti PE, Ikawa M.
CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in mice.
Biol Reprod. 2020 Oct 29;103(5):912-914. 

*Liu C, *Miyata H, *Gao Y, *Sha Y, *Tang S, *Xu Z, Whitfield M, Patrat C, Wu H, Dulioust E, Tian S, Shimada K, Cong J, Noda T, Li H, Morohoshi A, Cazin C, Kherraf ZE, Arnoult C, Jin L, He X, Ray PF, Cao Y, Touré A, Zhang F, Ikawa M. *:equal contribution.
Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility.
Am J Hum Genet. 2020 Aug 6;107(2):330-341. 

*Park S, *Shimada K, Fujihara Y, Xu Z, Shimada K, Larasati T, Pratiwi P, Matzuk RM, Devlin DJ, Yu Z, Garcia TX, Matzuk MM, 

Ikawa M. *:equal contribution.
CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity.
Biol Reprod. 2020 Aug 4;103(2):195-204. 

Miyata H, Morohoshi A, Ikawa M.
Analysis of the sperm flagellar axoneme using gene-modified mice.
Exp Anim.  2020 Nov 12;69(4):374-381.

*Sun J, *Lu Y, Nozawa K, Xu Z, Morohoshi A, Castaneda JM, Noda T, Miyata H, Abbasi F, Shawki HH, Takahashi S, Devlin DJ, Yu Z, Matzuk RM, Garcia TX, Martin MM, Ikawa M. *:equal contribution.
CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproduction.
Biol Reprod. 2020 Aug 4;103(2):183-194. 

*Xu Z, *Miyata H, Kaneda Y, Castaneda JM, Lu Y, Morohoshi A, Yu Z, Matzuk MM, Ikawa M.
CIB4 Is Essential for the Haploid Phase of Spermatogenesis in Mice.
Biol Reprod. 2020 Aug 4;103(2):235-243. 

*Larasati T, *Noda T, Fujihara Y, Shimada K, Tobita T, Yu Z, Matzuk MM, Ikawa M. *:equal contribution
Tmprss12 is required for sperm motility and uterotubal junction migration in mice.
Biol Reprod. 2020 Aug 4;103(2):254-263.

*Noda T, *Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk MM,  Ikawa M.*:equal contribution
Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
PNAS.2020;117(21):11493‐11502.

*Kobayashi K, *Endo T, Matsumura T, Lu Y, Yu Z, Matzuk MM, Ikawa M.*:equal contribution
Prss55 but Not Prss51 Is Required for Male Fertility in Mice.
Biol Reprod. 2020 Aug 4;103(2):223-234. 

Fujihara Y, Lu Y, Noda T, Oji A, Larasati T, Kojima-Kita K, Yu Z, Matzuk RM, Matzuk MM, Ikawa M
Spermatozoa Lacking Fertilization Influencing Membrane Protein (FIMP) Fail to Fuse With Oocytes in Mice.
PNAS. 2020 Apr 28;117(17):9393-9400.

*Abbasi F, *Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, Ikawa M. *:equal contribution
CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice
Cells. 2020;9(4):E821. Published 2020 Mar 28.

Miyata H, Shimada K, Morohoshi A, Oura S, Matsumura T, Xu Z, Oyama Y, Ikawa M.
Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa.
FASEB J. 2020 Apr;34(4):5389-5400.

Kiyozumi D, Mori M, Kodani M, Ikawa M.
Genetic mutation of Frem3 does not cause Fraser syndrome in mice.
Exp Anim. 2020 Jan 29;69(1):104-109.

Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, Ikawa M.
Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice.
PLoS Genet. 2020 Jan 21;16(1):e1008585.

Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, Ikawa M.
TESMIN, METALLOTHIONEIN-LIKE 5, is required for spermatogenesis in mice.
Biol Reprod. 2020;102(4):975–983.

2019

Noda T, Sakurai N, Nozawa K, Kobayashi S, Devlin DJ, Matzuk MM, Ikawa M.

Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice. 

Andrology. 2019; 7(5): 644–653 

 

Tobita T, Kiyozumi D, Muto M, Noda T, Ikawa M.

Lvrn expression is not critical for mouse placentation. 

J Reprod Dev. 2019; 65(3): 239–244.

 

Shimada K, Kato H, Miyata H, Ikawa M.

Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis. 

J Reprod Dev. 2019; 65(2): 155–162.

 

Matsumura T, Endo T, Isotani A, Ogawa M, Ikawa M.

An Azoospermic Factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility.

 J Reprod Dev. 2019;65(2):121-128.

Senoo M, Takijiri T, Yoshida N, Ozawa M, Ikawa M.

PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia.

J Reprod Dev 2019;65:37–46.

Komura S, Ito K, Ohta S, Ukai T, Kabata M, Itakura F, Semi F, Matsuda Y, Hashimoto K, Shibata H, Sone M, Jo N, Sekiguchi K, Ohno T, Akiyama H, Shimizu K, Woltjen K, Ozawa M, Toguchi J, Yamamoto T, Yamada Y.

Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas.

Nat Commun 2019;10:3999.

Sasanuma H, Ozawa M, Yoshida N.

RNA binding protein Ptbp1 is essential for BCR-mediated antibody production.

Int Immunol 2019;31:158-166.

*Matsumura T, *Noda T, Muratani M, Okada R, Yamane M, Isotani A, Kudo T, Takahashi S, Ikawa M.
*: equal contribution
Male mice, caged in the International Space Station for 35 days, sire healthy offspring.
Sci Rep. 2019;9(1):13733.

Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi D, Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX, 

Ikawa M.

CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice.
Biol Reprod. 2019;101(2):501-511.

Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, Ikawa M.

Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity.

Biol Reprod.2019 Apr 19;100(4):1035-1045

Oura S, Miyata H, Noda T, Shimada K, Matsumura T, Morohoshi A, Isotani A, Ikawa M.
Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice.
Exp Anim. 2019 Feb 26;68(1):25-34.

2018

Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, Ikawa M. Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity.Biol Reprod. 2018 Nov 18.

 

Fujihara Y, Oji A, Kojima-Kita K, Larasati T, Ikawa M. Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice.J Cell Sci. 2018 Oct 8;131(19).

 

Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, Ikawa M. RSPH6A is required for sperm flagellum formation and male fertility in mice.J Cell Sci. 2018 Oct 11;131(19).

 

Oura S, Miyata H, Noda T, Shimada K, Matsumura T, Morohoshi A, Isotani A, Ikawa M. Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice. Exp Anim. 2018 Aug 9. 

 

Nozawa K, Satouh Y, Fujimoto T, Oji A, Ikawa M. Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice. Sci Rep. 2018 Jan 22;8(1):1315.

Sasanuma S, Ozawa M, Yoshida N. The RNA binding protein Ptbp1 is essential for BCR-mediated antibody production. Int Immunol (accepted)
 

2017

Fujihara Y, Oji A, Larasati T, Kojima-Kita K, Ikawa M

Human globozoospermia-related gene Spata16 is required for spermformation revealed by CRISPR/Cas9-mediated mouse models.

International Journal of Molecular Sciences, 18(10): 2208 (2017)

Castaneda JMHua RMiyata HOji AGuo YCheng Y, Zhou TGuo XCui YShen BWang ZHu ZZhou ZSha JPrunskaite-Hyyrylainen RYu ZRamirez-Solis RIkawa MMatzuk MMLiu M. TCTE1 is a conserved component of the dynein regulatory complex and is required for motilityand metabolism in mouse spermatozoa.Proc Natl Acad Sci U S A.  pii: 201621279 (2017)

Tribulo P, Moss JI, Ozawa M, Jiang Z, Tian C, Hansen PJ. WNT regulation of embryonic development likely involves pathways independent of nuclear CTNNB1. Reproduction 153:405-419 (2017)

2016

Zhang X, Chen H, Wu X, Kodani A, Fan J, Doan R, Ozawa M, Ma J, Yoshida N, Reiter JF, Black DL, Kharchenko PV, Sharp PA, Walsh CA. Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex. Cell 166:1147-1162 (2016)

Ozawa M, Sakatani M, Dobbs K, Kannampuzha-Francis J, Hansen PJ. Regulation of gene expression in the bovine blastocyst by colony stimulating factor 2. BMC Research Notes 9:250 (2016)

Ozawa M, Fukuda T, Sakamoto R, Honda H, Yoshida N. The Histone Demethylase FBXL10 Regulates the Proliferation of Spermatogonia and Ensures Long-Term Sustainable Spermatogenesis in Mice. Biol Reprod 94:1-11 (2016)

Yang QE, Ozawa M, Zhang K, Johnson S, Ealy AD. The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development. Reprod Fertil Dev 28:482-490 (2016)

2014

Ozawa M, Kawakami E, Sakamoto R, Shibasaki T, Goto A, Yoshida N. Development of FGF2-dependent pluripotent stem cells showing naive state characteristics from murine preimplantation inner cell mass. Stem Cell Res 13:75-87 (2014)

Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N. The histone demethylases Fbxl11 plays an essential role in embryonic development by repressing cell-cycle regulators. Mech Devel 135:31-42 (2014)

Nakai M, Ozawa M, Maedomari N, Noguchi J, Kaneko H, Ito J, Onishi A, Kashiwazaki N, Kikuchi K. Delay in cleavage of porcine embryos after intracytoplasmic sperm injection (ICSI) shows poorer embryonic development. J Reprod Dev 60:256-259 (2014)

2013

Ozawa M, Yang QE, Ealy AD. The expression of fibroblast growth factor receptors during early bovine conceptus development and pharmacological analysis of their actions on trophoblast growth in vitro. Reproduction 145:191-201 (2013)

Sakatani M, Bonilla L, Dobbs KB, Block J, Ozawa M, Shanker S, Yao J, Hansen PJ. Changes in the transcriptome of morula-stage bovine embryos caused by heat shock: relationship to developmental acquisition of thermotolerance. Reprod Biol Endocrinol 11:3 (2013)

Dobbs KB, Khan FA, Sakatani M, Moss JI, Ozawa M, Ealy AD, Hansen PJ. Regulation of pluripotency of inner cell mass and growth and differentiation of trophectoderm of the bovine embryo by colony stimulating factor 2. Biol Reprod 89:1-10 (2013)

2012

Ozawa M, Sakatani M, Yao J, Shanker S, Yu F, Yamashita R, Wakabayashi S, Nakai K, Dobbs KB, Sudano MJ, Farmerie WG, Hansen PJ. Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst. BMC Dev Biol 12:33 (2012)

Ozawa M, Sakatani M, Hankowski KE, Terada N, Dobbs KB, Hansen PJ. Importance of culture conditions during the morula-to-blastocyst period on capacity of inner cell-mass cells of bovine blastocysts for establishment of self-renewing pluripotent cells. Theriogenology 78:1243-1251 (2012)

Nunomura S, Shimada S, Kametani Y, Yamada Y, Yoshioka M, Suemizu H, Ozawa M, Itoh T, Kono A, Suzuki R, Tani K, Ando K, Yagita H, Ra C, Habu S, Satake M, Sasaki E. Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset). Int Immunol 24:593-603 (2012)

2011

Bonilla AQ, Oliveira LJ, Ozawa M, Newsom EM, Lucy MC. Hansen PJ. Developmental changes in thermoprotective actions of insulin-like growth factor-1 on the preimplantation bovine embryo. Mol Cell Endocrinol 332:170-179 (2011)

Bonilla AQ, Ozawa M, Hansen PJ. Timing and dependence upon mitogen-activated protein kinase signaling for pro-developmental actions of insulin-like growth factor 1 on the preimplantation bovine embryo. Growth Horm IGF Res 2:107-111 (2011)

Ozawa M, Hansen PJ. A novel method for purification of inner cell mass and trophectoderm cells from blastocysts using magnetic activated cell sorting. Fertil Steril 95:799-802 (2011)

Thompson IM, Ozawa M, Bubolz JW, Yang Q, Dahl GE. Bovine luteal prolactin receptor expression: potential involvement in regulation of progesterone during the estrous cycle and pregnancy. J Anim Sci 89:1338-1346 (2011)

Yang QE, Fields SD, Zhang K, Ozawa M, Johnson SE, Ealy AD. Fibroblast Growth Factor 2 Promotes Primitive Endoderm Development in Bovine Blastocyst Outgrowths. Biol Reprod 85:946-953 (2011)

2010

Dang-Nguyen TQ, Kikuchi K, Somfai, Ozawa M, Nakai M, Maedomari Viet-Linh N, Kanai Y, Nguyen BX, Nagai T. Evaluation of developmental competence of in vitro-produced porcine embryos based on the timing, pattern and evenness of the first cleavage and the onset of the second cleavage. J Reprod Dev 56:593-600 (2010)

Maedomari N, Kikuchi K, Nagai T, Fahrudin M, Kaneko H, Noguchi J, Nakai M, Ozawa M, Somfai T, Nguyen LV, Ito J, Kashiwazaki N. Nuclear Replacement of In Vitro-Matured Porcine Oocytes by a Serial Centrifugation and Fusion Method. Reprod Domest Anim 45:659-665 (2010)

Nakai M, Kaneko H, Somfai T, Maedomari N, Ozawa M, Noguchi J, Ito J, Kashiwazaki N, Kikuchi K. Production of viable piglets for the first time using sperm derived from ectopic testicular xenografts. Reproduction 139:331–335 (2010)

Ozawa M, Nagai T, Somfai T, Nakai M, Maedomari N, Miyazaki H, Kaneko H, Noguchi J, Kikuchi K. Cumulus cell-enclosed oocytes acquire a capacity to synthesize GSH by FSH stimulation during in vitro maturation in pigs. J Cell Physiol 222:294-301 (2010)

Somfai T, Noguchi J, Kaneko H, Nakai M, Ozawa M, Kashiwazaki N, Egerszegi I, Rátky J, Nagai T, Kikuchi K. Production of good-quality porcine blastocysts by in vitro fertilization of follicular oocytes vitrified at the germinal vesicle stage. Theriogenology 73:147-156 (2010)